| 产品编号 | bsm-52473R |
| 英文名称 | Cyclophilin B Recombinant Rabbit mAb, Loading Control |
| 中文名称 | 亲环蛋白（亲环素）PPIB（内参）重组兔单抗 |
| 别 名 | Cphn 2; Cphn2; CyP 20b; CYP S1; CYP-S1; CYPB; MGC14109; MGC2224; peptidyl prolyl cis trans isomerase B; Peptidyl prolyl cis trans isomerase B precursor; Peptidyl-prolyl cis-trans isomerase B precursor; Peptidylprolyl isomerase B; PPIase; PPIB; Rotamase; S |
| 产品类型 | 内参抗体 重组兔单抗 |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 4A10 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 20 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Cyclophilin B: C terminus |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The cyclophilins are a conserved class of proteins that bind the immunosuppressive drug cyclosporin A (CsA) with high affinity. CsA blocks helper T-cell activation at a step between T-cell receptor stimulation and the transcriptional activation of cytokine genes. Cyclophilins from many species possess peptidyl-prolyl cis-trans isomerase (PPIase) activity that is blocked by CsA and therefore may be relevant in CsA-mediated immunosuppression. Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Subcellular Location: Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. DISEASE: Defects in PPIB are the cause of osteogenesis imperfect ype 9 (OI9) [MIM:259440]. OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients. Similarity: Belongs to the cyclophilin-type PPIase family. PPIase B subfamily. Contains 1 PPIase cyclophilin-type domain. SWISS: P23284 Gene ID: 5479 Database links: Entrez Gene: 5479 Human Entrez Gene: 19035 Mouse Omim: 123841 Human SwissProt: P23284 Human SwissProt: P24369 Mouse Unigene: 434937 Human Unigene: 335249 Mouse Unigene: 1893 Rat |
| 产品图片 |
Sample:
Lane 1: Placenta (Rat) Lysate at 40 ug
Lane 2: Liver (Mouse) Lysate at 40 ug
Lane 3: Kidney (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: Liver (Rat) Lysate at 40 ug
Lane 6: Kidney (Rat) Lysate at 40 ug
Lane 7: Hela (Human) Cell Lysate at 30 ug
Lane 8: A431 (Human) Cell Lysate at 30 ug
Lane 9: HepG2 (Human) Cell Lysate at 30 ug
Lane 10: Huvec (Human) Cell Lysate at 30 ug
Lane 11: U937 (Human) Cell Lysate at 30 ug
Primary: Anti- Cyclophilin B (bsm-52473R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 18 kD
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: Thyroid gland (Rat) Lysate at 40 ug
Lane 5: Thyroid gland (Mouse) Lysate at 40 ug
Lane 6: Placenta (Rat) Lysate at 40 ug
Lane 7: Placenta (Mouse) Lysate at 40 ug
Lane 8: Cerebrum (Rat) Lysate at 40 ug
Lane 9: Cerebrum (Mouse) Lysate at 40 ug
Lane 10: Liver (Rat) Lysate at 40 ug
Lane 11: Liver (Mouse) Lysate at 40 ug
Lane 12: Kidney (Rat) Lysate at 40 ug
Lane 13: Kidney (Mouse) Lysate at 40 ug
Lane 14: Huvec (Human) Cell Lysate at 30 ug
Primary: Anti-Cyclophilin B (bsm-52473R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclophilin B(Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52473R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclophilin B(Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52473R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
