| 产品编号 | bs-46009P |
| 英文名称 | Recombinant SARS-Cov-2 Spike RBD protein, C-mFc (HEK293) |
| 中文名称 | 重组SARS冠状病毒2 Spike RBD蛋白 |
| 别 名 | coronavirus spike Protein, 2019-nCoV; cov spike Protein, 2019-nCoV; novel coronavirus RBD Protein, 2019-nCoV; novel coronavirus s1 Protein, 2019-nCoV; novel coronavirus spike Protein, 2019-nCoV; RBD Protein, 2019-nCoV; S1 Protein, 2019-nCoV; pike RBD Prot 新冠病毒S蛋白RBD; 新冠S蛋白RBD; SARS-CoV-2 S蛋白RBD; |
 |
Specific References (1) | bs-46009P has been referenced in 1 publications.
[IF=6.784] Curreli F et al. Stapled Peptides Based on Human Angiotensin-Converting Enzyme 2 (ACE2) Potently Inhibit SARS-CoV-2 Infection In VitromBio.2020 Dec 11;11(6):e02451-20. other ;
|
| 理论分子量 | 14.3 kDa |
| 检测分子量 | 65 |
| 性 状 | Lyophilized |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | SARS-Cov-2 |
| 序 列 | 319-532/1273 |
| 纯 度 | >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC |
| 纯化方法 | AC |
| 内毒素 | <1.0 EU/μg as determined by LAL |
| 表达系统 | HEK293 cell |
| 活性 | Not tested |
| 标签 | C-mFc |
| 缓 冲 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| 产品图片 |
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
